Promega Corporation

P450-Glo™ CYP450 Screening Systems

The P450-Glo™ Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays. The systems include a membrane preparation containing recombinant human cytochrome P450 enzyme, a luminogenic cytochrome P450 substrate appropriate for the enzyme, an NADPH Regeneration System, reaction buffer, Luciferin Detection Reagent and Luciferin-Free Water. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450 and P450 reductase (and cytochrome b5 for CYP2C9 and CYP3A4). The P450-Glo™ Screening Systems also contain a membrane fraction devoid of c...

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  • Prices valid for customers of Promega Korea Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

P450-Glo™ CYP3A4 Screening System with Luciferin-IPA

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P450-Glo™ CYP3A4 Screening System with Luciferin-IPA

 Components

  • Reconstitution Buffer with esterase

    V144B1 x 50ml
  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP3A4 (1pmol/μl) + Reductase + b5

    V480B1 x 100μl
  • Luciferin-IPA

    V840B1 x 60μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9920 ₩ 1,423,000 Add to cart

P450-Glo™ CYP3A4 Screening System (Luciferin-PPXE)
DMSO-Tolerant Assay

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P450-Glo™ CYP3A4 Screening System (Luciferin-PPXE)
DMSO-Tolerant Assay

 Components

  • Luciferin-Free Water

    V348A1 x 50ml
  • 2M Tris-HCl (pH 7.5), 20X

    V349A1 x 1ml
  • Potassium Phosphate Buffer, 1M

    V446A2 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP3A4 (1pmol/μl) + Reductase + b5

    V480A1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
  • Luciferin-PPXE

    V997A2 x 15μl
1,000 assays
- V9910 ₩ 1,423,000 Add to cart

P450-Glo™ CYP1A2 Screening System

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P450-Glo™ CYP1A2 Screening System

 Components

  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP1A2 (1pmol/μl) + Reductase

    V477A1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-ME, 5mM

    V862B1 x 1ml
  • Reconstitution Buffer

    V865B1 x 50ml
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9770 ₩ 1,423,000 Add to cart

P450-Glo™ CYP2C9 Screening System

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P450-Glo™ CYP2C9 Screening System

 Components

  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP2C9 (1pmol/μl) + Reductase + b5

    V479A1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-H, 5mM

    V864B1 x 1ml
  • Reconstitution Buffer

    V865B1 x 50ml
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9790 ₩ 1,423,000 Add to cart

P450-Glo™ CYP3A4 Screening System

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P450-Glo™ CYP3A4 Screening System

 Components

  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A2 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP3A4 (1pmol/μl) + Reductase + b5

    V480A2 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
  • Luciferin-BE, 5mM

    V866B1 x 500μl
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9800 ₩ 1,423,000 Add to cart

P450-Glo™ CYP2C19 Screening System

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P450-Glo™ CYP2C19 Screening System

 Components

  • Reconstitution Buffer with esterase

    V144B1 x 50ml
  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP2C19 (1pmol/μl) + Reductase + b5

    V488A1 x 250μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-H EGE

    V867A2 x 123μg
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9880 ₩ 1,423,000 Add to cart

P450-Glo™ CYP2D6 Screening System

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P450-Glo™ CYP2D6 Screening System

 Components

  • Reconstitution Buffer with esterase

    V144B1 x 50ml
  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP2D6 (1pmol/μl) + Reductase

    V489A1 x 250μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-ME EGE

    V869B1 x 900μg
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9890 ₩ 1,423,000 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

NADPH Regeneration System

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NADPH Regeneration System

 Components

  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9510 ₩ 191,000 Add to cart

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Storage Conditions

Store the CYP1A2, CYP2C9 and CYP3A4 membranes at –70°C. Cytochrome P450 may lose activity with repeated freeze-thaw cycles. Avoid multiple freeze-thaw cycles by dispensing the CYP1A2, CYP2C9 and CYP3A4 membranes into single-use aliquots (e.g., 50μl for 96 reactions). Store aliquots at –70°C. All other components can be stored at –20°C or –70°C and protected from light. The reconstituted Luciferin Detection Reagent can be stored at –20°C for up to 3 months. For convenience, the reconstituted Luciferin Detection Reagent can be stored at room temperature (approximately 23°C) without loss of activity for 24 hours or at 4°C for 1 week. Avoid multiple freeze-thaw cycles of all components.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V9920, V9910, V9770, V9790, V9800, V9880, V9890, V9510 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V9920, V9910, V9770, V9790, V9800, V9880, V9890 U.S. Pat. No. 7,692,022 and other patents pending.

V9920, V9910, V9770, V9790, V9800, V9880, V9890 Patent Pending.

V9920, V9910, V9770, V9790, V9800, V9880, V9890 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V9920, V9910, V9770, V9790, V9800, V9880, V9890 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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