Promega Corporation

Caspase-Glo® 2 Assay Systems

The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity. Caspase-2 is a member of the cysteine aspartic acid-specific protease family. The Caspase-Glo® 2 Assay provides a luminogenic substrate (Z-VDVAD-aminoluciferin) in a reagent optimized for caspase-2 and luciferase activity. A single reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present. The assay system may be used with purified enzyme preparations and i...

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Caspase-Glo® 2 Assay

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Caspase-Glo® 2 Assay

 Components

  • Caspase-Glo® 2 Buffer

    G092A1 x 10ml
  • Z-VDVAD-Glo™ Substrate

    G093A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10ml
- G0940 ₩ 578,000 Add to cart

Caspase-Glo® 2 Assay

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Caspase-Glo® 2 Assay

 Components

  • Caspase-Glo® 2 Buffer

    G092B1 x 50ml
  • Z-VDVAD-Glo™ Substrate

    G093B1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G0941 Please Enquire


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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Sensitivity of the Caspase-Glo 2 Assay compared to fluorescent assays.Figure 1. Sensitivity of the Caspase-Glo® 2 Assay compared to fluorescent assays.
Caspase-2 cleavage of the luminogenic substrate containing the VDVAD sequence.Figure 2. Caspase-2 cleavage of the luminogenic substrate containing the VDVAD sequence.

Use Restrictions

G0940, G0941 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G0940, G0941 U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and other patents and patents pending.

G0940, G0941 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G0940, G0941 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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