CellTiter-Blue® Cell Viability Assay

The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer.

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The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer.

Features - Benefits

• Save Time: The homogeneous, add-incubate-measure format reduces the number of handling steps.
• Perform More Than One Assay on the Same Sample: The system can be multiplexed with other assay methods such as the Apo-ONE® Homogeneous Caspase-3/7 Assay (Cat.# G7790) or the Caspase-Glo® Assays (Cat.# G8090, G8200, G8210) for detecting apoptosis.
• Gain Flexibility: The CellTiter-Blue® Assay has an excellent Z´ factor and offers more flexibility in assay incubation times compared to other resazurin-based assays.
• Safe: The reagent is generally nontoxic to cells, allowing extended incubation periods in some situations. Requires no scintillation cocktail, radioactive waste disposal (unlike [³H]-thymidine incorporation assays) or hazardous solvents (as required for MTT tetrazolium-based assays).
• Adapt to Your Throughput Needs: The reagent is designed to provide sufficient volumes for accurate pipetting into 96- or 384-well formats. Convenient product sizes available for high-throughput screening.
• Automate This Assay: Validated automated methods available at: www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Cell proliferation.
• Cytotoxicity.
• Cell viability.

For more information, see the Protocols & Applications Guide.

Notes

Each G8080 system provides sufficient reagents to perform 1,000 assays in a 96-well format or 4,000 assays in a 384-well format when the recommended volumes are used. Likewise, each G8081 system provides sufficient reagents to perform 5,000 and 20,000 assays; each G8082 system provides sufficient reagents to perform 50,000 and 200,000 assays.

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