Promega Corporation

PowerPlex® Fusion System

The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This five-color system allows co-amplification and fluorescent detection of the 13 core CODIS (US) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the 12 core European Standard Set loci (TH01, vWA, FGA, D21S11, D3S1358, D8S1179, D18S51, D10S1248, D22S1045, D2S441, D1S1656 and D12S391) and Amelogenin for gender determination. In addition, the male-specific DYS391 locus is included to identify null Y ...

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  • Prices valid for customers of Promega Korea Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

PowerPlex® Fusion System

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PowerPlex® Fusion System

 Components

  • 2800M Control DNA

    DD710A1 x 25μl
  • CC5 Internal Lane Standard 500

    DG152A2 x 300μl
  • PowerPlex® Fusion Allelic Ladder Mix

    DG381B1 x 100μl
  • PowerPlex® Fusion 5X Primer Pair Mix

    DK236B1 x 1ml
  • PowerPlex® Fusion 5X Master Mix

    DP207A1 x 1ml
  • Water, Amplification Grade

    DW099A5 x 1,250μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Already a Helix Customer?

Request to add this product to your Helix »


200 reactions
- DC2402 Please Enquire

PowerPlex® Fusion System

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PowerPlex® Fusion System

 Components

  • 2800M Control DNA

    DD710A1 x 25μl
  • CC5 Internal Lane Standard 500

    DG152A8 x 300μl
  • PowerPlex® Fusion Allelic Ladder Mix

    DG381B4 x 100μl
  • PowerPlex® Fusion 5X Primer Pair Mix

    DK236B4 x 1ml
  • PowerPlex® Fusion 5X Master Mix

    DP207A4 x 1ml
  • Water, Amplification Grade

    DW099A10 x 1,250μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


800 reactions
- DC2408 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

PowerPlex® 5-Dye Matrix Standards, 3100/3130

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PowerPlex® 5-Dye Matrix Standards, 3100/3130

 Components

  • CC5 Matrix, 5-Dye (3100/3130)

    DG154A1 x 25μl
  • CXR-ET Matrix, 5-Dye (3100/3130)

    DG155A1 x 25μl
  • TMR-ET Matrix, 5-Dye (3100/3130)

    DG156A1 x 25μl
  • JOE Matrix, 5-Dye (3100/3130)

    DG157A1 x 25μl
  • Fluorescein Matrix, 5-Dye (3100/3130)

    DG158A1 x 25μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
25μl
(each dye)
- DG4700 Please Enquire

2800M Control DNA

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2800M Control DNA

 Components

  • 2800M Control DNA

    DD710A1 x 25μl
25μl
10ng/μl DD7101 Please Enquire

CC5 Internal Lane Standard 500

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CC5 Internal Lane Standard 500

 Components

  • CC5 Internal Lane Standard 500

    DG152A1 x 300μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


300μl
- DG1521 Please Enquire

Water, Amplification Grade

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Water, Amplification Grade

 Components

  • Water, Amplification Grade

    DW099A5 x 1,250μl
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  • This product can be added to a

  • Helix Cabinet, Freezer, Refrigerator

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


6,250μl
(5 × 1,250μl)
- DW0991 ₩ 49,000 Add to cart

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Storage Conditions

Store kit at –20°C. Upon receipt, move 2800M Control DNA to 4°C storage.

For product intended use please see Patents & Disclaimers tab.

10910MA_550pxFigure 1. The 24 loci included in the PowerPlex® Fusion System.

This system allows co-amplification of Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317 and Penta E labeled with fluorescein; D16S539, D18S51, D2S1338, CSF1PO and Penta D labeled with JOE; TH01, vWA, D21S11, D7S820, D5S818, TPOX and DYS391 labeled with TMR-ET; and D8S1179, D12S391, D19S443, FGA and D22S1045 labeled with CXR-ET. The CC5 Internal Lane Standard 500 (CC5 ILS 500) is labeled with CC5 dye and contains 21 DNA fragments of 60, 65, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 and 500 bases in length.

This system allows co-amplification of Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317 and Penta E labeled with fluorescein; D16S539, D18S51, D2S1338, CSF1PO and Penta D labeled with JOE; TH01, vWA, D21S11, D7S820, D5S818, TPOX and DYS391 labeled with TMR-ET; and D8S1179, D12S391, D19S443, FGA and D22S1045 labeled with CXR-ET. The CC5 Internal Lane Standard 500 (CC5 ILS 500) is labeled with CC5 dye and contains 21 DNA fragments of 60, 65, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 and 500 bases in length.

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10911MA_250pxFigure 2. Amplification of 100pg of human DNA using 30 cycles and the PowerPlex® Fusion System.

Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

11018MA_250pxFigure 3. The PowerPlex® Fusion System demonstrates superior sensitivity, generating full profiles with as little as 100pg input DNA.
10912MB_550Figure 4. Human genomic DNA (500pg) was amplified with each listed kit in the presence of common PCR inhibitors.

Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection). Percentage of alleles detected are indicated for each system.

Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection). Percentage of alleles detected are indicated for each system.

/~/media/images/resources/figures/10900-10999/10912mb_550px.jpg?la=ko-KR
10913MA_275pxFigure 5. One 1.2mm buccal punch of an S&S903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolution Kit Technical Manual TMD038.
10914MA_250pxFigure 6. A swab was pretreated with SwabSolution™ Reagent.

Following incubation, 2µl of extract was added to the PCR amplification mix and amplified as described in the PowerPlex® Fusion System Technical Manual TMD039.

10915MA_250pxFigure 7. Direct amplification of one 1.2mm FTA® card punch containing blood using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039.
10916MB_250pxFigure 8. Direct amplification of DNA from two 1.2mm FTA® card punches from a buccal sample using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039.

Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Use Restrictions

DC2402, DC2408, DG4700, DD7101, DG1521, DW0991 Not For Medical Diagnostic Use.

Patents - Disclaimers

DC2402, DC2408 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

DC2402, DC2408 U.S. Pat. No. 6,238,863 and other patents and patents pending.

DC2402, DC2408 U.S. Pat. Nos. 5,843,660, 6,479,235, 6,221,598 and 7,008,771, Australian Pat. No. 724531, Canadian Pat. Nos. 2,118,048 and 2,251,793 and other patents and patents pending.

DC2402, DC2408, DG4700 This product or portions thereof is manufactured and sold under license from GE Healthcare under Australia Pat. No. 692230, Austria Pat. No. E236994, Belgium Pat. No. 0743987, Canada Pat. No. 2231475, EP Pat. Nos. 0743987 and 0851867, France Pat. Nos. 0743987 and 0851867, Germany Pat. Nos. 19581489, 69530286.8 and 0851867, Italy Pat. Nos. 0743987 and 0851867, Japan Pat. No. 3066984, Liechtenstein Pat. Nos. 0743987 and 0851867, Netherlands Pat. Nos. 0743987 and 0851867, Spain Pat. Nos. 2197193 and 2173310, Sweden Pat. Nos. 0743987 and 0851867, Switzerland Pat. Nos. 0743987 and 0851867, United Kingdom Pat. Nos. 0743987 and 0851867, U.S. Pat. Nos. 5,654,419, 5,688,648, 5,869,255, 6,177,247, 5,707,804, 6,028,190, 6,544,744, 7,015,000 and 5,728,528 and other pending and foreign patent applications.

End User Terms and Conditions

Acceptance. These terms and conditions shall govern the purchase, use, transfer and acceptance of the products described in the purchase order, quotation or invoice, which products are sold and distributed by Promega to the buyer/transferee of such products (the "End User"). The transfer/sale of products to the End User is expressly conditional upon End User's acceptance of these terms and conditions.

Restrictions on Use. End Users are specifically not authorized to and are forbidden from reselling, transferring or distributing any products either as a stand alone product or as a component of another product. The right to use the products does not, in and of itself, include or carry any right of the End User to any GE Healthcare Bio-Sciences Corp.'s technology or intellectual property other than expressly provided herein. End Users may not use sequence(s) in an attempt to reverse engineer parameters of any of GE Healthcare Bio-Sciences Corp. proprietary products or services.

Disclaimer of Warranties. GE Healthcare Bio-Sciences Corp. provides no warranties to end user (statutory or implied), including without limitation, as to product quality, condition, description, merchantability or fitness for a particular purpose, and all such warranties are hereby expressly disclaimed. GE Healthcare Bio-Sciences Corp. hereby expressly disclaims any warranty regarding results obtained through the use of the products, including without limitation any claim of inaccurate, invalid or incomplete results.

Exclusion of Liability. GE Healthcare Bio-Sciences Corp. and its affiliates shall have no liability to an End User, including, without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise.

DC2402, DC2408, DG4700 TMR-ET, CXR-ET and CC5 dyes are proprietary.

DC2402, DC2408 Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

DC2402, DC2408 STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany.

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