Anti-Human BDNF pAb

BDNF, a 27kDa homodimer originally derived from human brain, shares high sequence homology with NGF, NT-3 and NT-4/5 and influences many neuron types in the CNS. Anti-Human BDNF pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for BDNF.

Expand to Read More »

BDNF, a 27kDa homodimer originally derived from human brain, shares high sequence homology with NGF, NT-3 and NT-4/5 and influences many neuron types in the CNS. Anti-Human BDNF pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for BDNF.

Features - Benefits

• Immunogen: Human recombinant BDNF.
• Antibody Form: Chicken IgY, provided at 0.5mg/ml in 0.1M NaCl, 0.01M K₂HPO₄ and 50μg/ml gentamicin.
• Specificity: Cross-reactive between mammalian species; does not cross-react with other neurotrophic factors.

Applications

(Suggested working concentration)

• Western blotting (1μg/ml).
• ELISA (1μg/ml).
• Immunocytochemistry (1–10μg/ml); methanol-fixed (1–10μg/ml).
• Immunohistochemistry (10–15μg/ml).
• Biological neutralization (10μg/ml).

For more information, see the Protocols & Applications Guide.

« Collapse Content