pGEM®-T Easy Vector Systems

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

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The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

Features - Benefits

• Flexibility: The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options to remove the insert with a single digest.
• Rapid Ligation: The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature.
• Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
• f1 Origin of Replication: Allows preparation of single-stranded DNA.

Applications

For more information, see the Protocols & Applications Guide.

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