Promega Corporation

Bisulfite Conversion for DNA Methylation Workflows

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Bisulfite Conversion Kit
Rapid Bisulfite Conversion with Minimal DNA Fragmentation

The MethylEdge™ System provides an efficient method to rapidly perform bisulfite conversion, giving you more intact, converted DNA for downstream applications. Use the MethylEdge™ System with other DNA Methylation Workflow reagents to perform sensitive DNA methylation analysis on many sample types.

Realize the full potential of your samples with the most effective bisulfite conversion system available. The MethylEdge™ Bisulfite Conversion System offers a robust system for rapidly performing bisulfite conversion with minimal DNA fragmentation.

  • Effective Conversion Reagents: High-efficiency DNA conversion from as little as 100pg input DNA 
  • Rapid Protocol: Time savings compared to other conversion systems
  • Intact DNA: Robust conversion of DNA with reduced DNA fragmentation
  • Room-Temperature, Ready-to-Use Reagents: Convenient system configuration allows room-temperature storage and minimal up-front preparation.

Completely convert your DNA in less than 2 hours and go directly into your downstream assay of choice. This all-inclusive kit provides you with more intact, usable, DNA for any application including qPCR, arrays, and sequencing.

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Schematic diagram of the MethylEdge™ Bisulfite Conversion System protocol. Convert DNA samples efficiently in less than 2 hours with minimal preprocessing steps.

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Degree of DNA fragmentation after conversion. DNA was purified from whole blood using the ReliaPrep™ Blood gDNA Miniprep System (1) or Maxwell® 16 LEV Blood Kit (2) and was converted using the Promega MethylEdge™ Bisulfite Conversion System (P) or a competing bisulfite conversion kit (Z or N). B = BenchTop 1kb DNA Ladder, D = nonconverted, purified DNA.

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The success of DNA methylation analysis experiments relies on quality genomic DNA as input. Promega offers high quality systems for manual and automated genomic DNA purification from many sample types.

Manual and High-Throughput Chemistries Hands-Free Automated Purification from 1-16 Samples
ReliaPrep Kit
ReliaPrep™ Genomic DNA Purification Systems offer comprehensive solutions for reliable purification of high quality gDNA. Kits include manual systems for purification from FFPE samples, blood and tissue and high-throughput gDNA purification from large volume blood samples.
Maxwell 16 Genomic Kit
The Maxwell® 16 Instrument and DNA Purification Kits provide automated DNA extraction from many sample types, including FFPE tissues. The convenient, hands-free extraction method frees up time and provides consistent yields of highly pure gDNA. See all the Maxwell® 16 Genomic DNA Purification Kits.
Accurate DNA quantization both pre- and post-bisulfite conversion is critical to experimental success. Promega offers robust, fluorescence-based detection systems for accurate quantitation.
Quantitation Instrument Chemistry
QuantiFluor™ dsDNA System- Offers sensitive detection of small amounts of double stranded DNA in solution upstream of bisulfite conversion.
QuantiFluor™ ssDNA System- Offers reliable, sensitive detection of bisulfite converted DNA in solution.

Amplify your bisulfite-converted DNA with the most robust, reliable amplification tools on the market. Whether your methylation assay requires real-time or end-point analysis, the GoTaq® Amplification Systems provide easy-to-use, sensitive reagents that meet your needs.

End-Point PCR Systems
GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers.
Dye-based Real-Time Systems GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad range detection for increased reliability, reproducibility and sensitivity. The system contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum efficiency, and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.

GoTaq® qPCR Master Mix

Probe-based Real-Time Systems The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). GoTaq® Probe qPCR Master Mix

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