The FluoroTect™ GreenLys in vitro Translation Labeling System is the first commercially available fluorescent technology for labeling in vitro translation products. The FluoroTect™ System uses a modified charged lysine transfer RNA (tRNA) labeled with the fluorophore, BODIPY®-FL. The modified lysine tRNA is used efficiently in both prokaryotic and eukaryotic in vitro translation systems. Data is obtained in a few minutes by the detection of the fluorescently labeled proteins "in-gel" with laser-based fluorescent gel scanning. This feature circumvents the multiple manipulations associated with traditional isotopic and nonisotopic incorporation and detection methodologies.
Promega Notes 77, 23–27.
Gary Kobs, Robin Hurst, Natalie Betz and Becky Godat