The HisLink™ 96 Protein Purification System allows efficient purification of polyhistidine-tagged proteins from 1ml of culture using a vacuum-based format. The system purifies polyhistidine-tagged proteins directly from bacterial, insect and mammalian cell culture without the need for prior processing of the culture or resin. Removing these prior processing steps results in a robust purification method that is easy to automate and adaptable to rapid screening processes.
Promega Notes 90, 15–18.
Laurie Engel, Rod Flemming, Tonny Johnson, Cristopher Cowan, Natalie Betz and Brian Almond