TNT® Coupled Wheat Germ Extract Systems Technical Bulletin

Literature # TB165

The TNT® Coupled Wheat Germ Extract Systems offers an alternative for eukaryotic in vitro translation: a one-tube, coupled transcription/translation system. The systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract-based translations commonly use RNA synthesized in vitro from a SP6, T3 or T7 RNA polymerase promoter. This entire process requires separate reactions with several steps between each reaction. The TNT® Systems bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT® System reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates.