TNT® T7 Quick for PCR DNA Technical Manual
Literature # TM235
The TNT® T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum expression of PCR templates. For most PCR templates, the TNT® T7 Quick for PCR DNA reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction. PCR products from amplification protocols and commercially available systems (e.g., Promega Access RT-PCR System [Cat.# A1250], Roche Diagnostics High Fidelity and Expand™ Long Template PCR Systems, and Invitrogen Platinum Taq) have been successfully tested. The system will work with any other comparable PCR amplification scheme. The PCR products may be added directly to the TNT® T7 Quick for PCR DNA reaction without purification.
To use TNT® T7 Quick for PCR DNA, a PCR fragment containing a T7 promoter is added to the TNT® T7 PCR Quick Master Mix and incubated for 60–90 minutes at 30°C. The synthesized proteins then can be analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography or phosphorimaging.
Printed in USA. Revised 12/12.