GoTaq® Long PCR Master Mix Technical Manual

Literature # TM359

GoTaq® Long PCR Master Mix is a ready-to-use solution for long PCR targets. The master mix contains everything needed to perform long PCR. Only the template and primers need to be added to amplify your target. The GoTaq® Long PCR Master Mix requires little or no reagent optimization and can amplify up to 30kb of genomic DNA and 40kb of lower-complexity targets such as plasmid and lambda DNA.

The GoTaq® Long PCR Master Mix uses a blend of hot-start, recombinant Taq DNA polymerase and a recombinant proofreading DNA polymerase. The recombinant Taq DNA polymerase is bound by proprietary antibodies that inhibit activity at lower temperatures. This allows reactions to be set up at room temperature. The polymerase activity is restored after an initial denaturation cycle at high temperature. In addition to Taq DNA polymerase, the GoTaq® Long PCR Master Mix contains a small amount of DNA polymerase with 3´-->5´ exonuclease (i.e., proofreading) activity.