Promega Corporation

Using Luciferase Assays to Study G-Protein-Coupled Receptor Pathways and Screen for...

Using Luciferase Assays to Study G-Protein-Coupled Receptor Pathways and Screen for GPCR Modulators

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Abstract

Luciferase reporter assay systems are successfully used in the drug discovery process against G-protein coupled receptors for both studying the GPCR signaling pathways and for screening for modulators of GPCRs. Here we describe a strategy for constructing stable cell lines expressing two luciferases on two plasmids that reduces assay noise and false positives when screening for downregulated responses (antagonist response of GPCR).

Cell Notes 13, 5–7.

Frank Fan, Alieen Paguio, Denise Garvin, and Keith V. Wood

Promega Corporation
Publication Date: 2005

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