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Brain Res. Bull. 52(2), 109-113. Glial cell line-derived neurotrophic factor in the rat pituitary gland 2000

Saland, L.C., Cunningham, L.A., Su, C., Morales, M., and Gaddy, J.

Notes: The Anti-Human GDNF pAb was used to detect GDNF in rat pituitaries by immunohistochemistry. To stain for GDNF alone, the rats were perfused with cold 4% paraformaldehyde, paraffin embedded, then sliced into 8µm sections. The sections were permeabilized with a brief trypsin treatment. The sections were blocked with normal donkey serum and incubated with the Anti-Human GDNF pAb (1:250 dilution) either overnight at ambient room temperature or for 48 hours at 4°C followed by colorimetric detection. For co-localization with other marker antibodies, the pituitaries were flash frozen and 10µm sections obtained. The frozen sections were fixed in 4% paraformaldehyde and permeablized with 0.3% Triton® X-100 in 5% normal goat serum. The sections were treated for 48 hours at 4°C in the presence of the Anti-Human GDNF pAb (1:250 dilution) in the goat serum/0.3% Triton® X-100 solution with accompanying antibodies. Actual levels of GDNF in the pituitaries were determined with the GDNF Emax® ImmunoAssay System. (0053)

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J. Neurosci. 20, 4686-700. Long-term rAAV-mediated gene transfer of GDNF in the rat Parkinson's model: intrastriatal but not intranigral transduction promotes functional regeneration in the lesioned nigrostriatal system. 2000

Kirik, D., Rosenblad, C., Bjorklund, A., and Mandel R.J.

Notes: An adeno-associated viral vector was used for long-term expression of GDNF in either the nigral DA neurons, in the striatal target cells, or in both of these structures. Only rats expressing GDNF in the striatum displayed behavioral recovery, and significant reinnervation of the lesioned striatum. GDNF expression was monitored using Promega's GDNF Emax® ImmunoAssay System. (2329)

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Science 290, 767-73. Neurodegeneration prevented by lentiviral vector delivery of GDNF in primate models of Parkinson's disease. 2000

Kordower, J.H., Emborg, M.E., Bloch, J., Ma, S.Y., Chu, Y., Leventhal, L., McBride, J., Chen, E.Y., Palfi, S., Roitberg, B.Z., Brown, W.D., Holden, J.E., Pyzalski, R., Taylor, M.D., Carvey, P., Ling, Z., Trono, D., Hantraye, P., Deglon, N., and Aebischer, P.

Notes: A lentiviral vector was used to express glial cell line-derived neurotrophic factor in nonhuman primate models of Parkinson's disease. GDNF expression in the substantia nigra seemed to reverse functional deficits and prevent nigrostriatal degeneration. GDNF expression in transduced and non-transduced cells of the rhesus monkey substantia nigra was quantitated using Promega's GDNF Emax® ImmunoAssay System. (2330)

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Neuroscience 90, 333-347. Alterations in expression of the neurotrophic factors glial cell line-derived neurotrophic factor, ciliary neurotrophic factor and brain-derived neurotrophic factor, in the target-deprived olfactory neuroepithelium 1999

Buckland, M.E., Cunningham, A.M.

Notes: The Anti-Human BDNF pAb, Anti-Rat CNTF pAb and Anti-Human GDNF pAb were used for immunohistochemical analysis of rat olfactory tissue. A lot of detail is provided for the staining. To verify the specificity of the antibodies, the antibodies were immunoabsorbed with their appropriate immunogen and all staining was abolished. (1370)

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Exp. Neurol. 145(2 Pt 1), 592-6. An acid-treatment method for the enhanced detection of GDNF in biological samples 1997

Okragly, A.J. and Haak-Frendscho, M.

Notes: The levels of GDNF, NGF, and NT-3 were quantitated in various mouse tissues, including spleen, muscle, heart, lung, liver, kidney, bladder, gastrointestinal tract, brain, and serum. Additional samples screened for NGF included pig, goat, horse, chicken, rabbit, sheep, and bovine serum and rat brain. Samples were untreated or acid treated by acidification to pH <3.0 followed by neutralization to pH 7.6. In all tissue samples and most serum samples, the detectable levels of GDNF, NGF and NT-3 increased up to 35-fold. It is thought that the acidification process promotes dissociation of receptors and associated proteins making the neurotrophins more accessible. Levels of GDNF, NGF, and NT-3 were determined using the GDNF, NGF, and NT-3 Emax ImmunoAssay Systems, respectively.  Please note that the coat antibody in the NGF Emax ImmunoAssay System was changed 12/2002 and may affect cross-reactivity. (3105)

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Blood 89, 2925-2937. Expression of the RET receptor tyrosine kinase and GDNFR-alpha in normal and leukemic human hematopoietic cells and stromal cells of the bone marrow microenvironment. 1997

Gattei, V., Celetti, A., Cerrato, A., Degan, M., De Iuliis, A., Rossi, F.M., Chiappetta, G., Consales, C., Improta, S., Zagonel, V., Aldinucci, D., Agosti, V., Santoro, M., Vecchio, G., Pinto, A. and Grieco, M.

Notes: Acute myeloid leukemia cells expressing RET were assayed for clonogenic growth of blast cells by GDNF with or without treatment with a phosphoinositol-specific phospholipase C. The GDNFR-alpha is phosphoinositol-linked protein. Cells were also treated with GDNF, reacted with the anti-GDNF antibody and reacted with the FITC-conjugated Anti-chicken IgY. The cells were analyzed by flow cytometry in the presence or absence of the phospholipase C. (1573)

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Development 124, 4077-4087. Glial-cell-line-derived neurotrophic factor is required for bud initiation from ureteric epithelium 1997

Sainio, K., Suvanto, P., Davies, J., Wartiovaara, J., Wartiovaara, K., Saarma, M., Arumae, U., Meng, X., Lindahl, M., Pachnis, V., Sariola, H.

Notes: The Anti-Human GDNF pAb was used to detect GDNF in homogenates of rat kidney grown in organ culture. Treatment of the cultures with chlorate dropped the GDNF levels below the detection level. The kidneys were homogenized in reducing SDS sample buffer. (0470)

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J. Neurosci. 17, 6504-6511. Targeted transduction of CNS neurons with adenoviral vectors carrying neurotrophic factor genes confers neuroprotection that exceeds the transduced population. 1997

Baumgartner, B.J. and Shine, H.D.

Notes: The GDNF Emax ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis. (1605)

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